Plasmid

Part:BBa_K4812024:Design

Designed by: Rashik Chand   Group: iGEM23_NYU-Abu-Dhabi   (2023-10-12)


pQE80L-Kan


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 88
    Illegal XbaI site found at 1163
    Illegal XbaI site found at 2453
    Illegal PstI site found at 179
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 88
    Illegal NheI site found at 307
    Illegal PstI site found at 179
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 88
    Illegal BamHI site found at 145
    Illegal XhoI site found at 1
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 88
    Illegal XbaI site found at 1163
    Illegal XbaI site found at 2453
    Illegal PstI site found at 179
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 88
    Illegal XbaI site found at 1163
    Illegal XbaI site found at 2453
    Illegal PstI site found at 179
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 2743


Design Notes

Suitable for expressing proteins with 6His tag for purification with Ni-NTA column.


Source

Qiagen

References